A multi-needle-assisted transformation of soybean cotyledonary node cells
نویسندگان
چکیده
منابع مشابه
High-throughput direct regeneration of soybean mutant and common lines from cotyledonary node
Direct regenerations by using mature cotyledonary node as a explants has been shown to be time-saving and convenient strategy for micropropagation of soybean. So we have evaluated regeneration protocol through single shoot using cotyledonary node as a rapid and efficient protocol for two soybean cultivars and one mutant line. Cotyledonary nodes explants obtained from 7-days-old in vitro</em...
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A reproducible and highly efficient protocol for genetic transformation mediated by Agrobacterium has been established for greengram (Vigna radiata L. Wilczek). Double cotyledonary node (DCN) explants were inoculated with Agrobacterium tumefaciens strain LBA 4404 harboring a binary vector pCAMBIA 2301 containing neomycin phosphotransferase (npt II) gene as selectable marker, β-glucuronidase (GU...
متن کاملhigh-throughput direct regeneration of soybean mutant and common lines from cotyledonary node
direct regenerations by using mature cotyledonary node as a explants has been shown to be time-saving and convenient strategy for micropropagation of soybean. so we have evaluated regeneration protocol through single shoot using cotyledonary node as a rapid and efficient protocol for two soybean cultivars and one mutant line. cotyledonary nodes explants obtained from 7-days-old in vitro seedlin...
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Lactuca sativa is widely used as a leafy vegetable belonging to the Asteraceae family. Cotyledonary leaves of in vitro lettuce seedlings were transformed by plasmids containing the GCHI gene. Also the effects of number of days of pre-cultivation and co-cultivation of explants were examined on the transformation frequency. After co-cultivation, the explants were transferred onto the regeneration...
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ژورنال
عنوان ژورنال: Biotechnology Letters
سال: 2006
ISSN: 0141-5492,1573-6776
DOI: 10.1007/s10529-006-9229-x